T.U.F.F Kitty is an episode off of T.U.F.F Puppy. It will contain gender swapped counterparts of other characters from the T.U.F.F Puppy series.
- Ashley Tisdale - Donnie Kitty
- Tom Kenny - Muttley Dogswell
- Lori Alan - Huberta "Boss" Dumbrowski
- Christine Cavanaugh - Kessie Weeks
- Algaia Mortcheva - Viridian Snaptrap
- Nancy Cartwright - Laurie
- Fran Drecsher - Francis
- June Foray - Camille Leon
- Kathryn Beaumont - Olive
- This might be the first time Ashley Tisdale will voice a Nickelodeon character.
- It is revealed that the name of the city in the episode is Petlantis.
- According to Butch Hartman, he announced that there could be another T.U.F.F Kitty episode when Dudley and the T.U.F.F agents meet Donnie.