Main CharactersConnor Retriever - As the main character of T.U.F.F. Puppies, Connor Retriever is ranked one of the best T.U.F.F. agents. Connor treats Seth and Dudley as if they were his brothers. He knows martial arts so it's not hard defeating Quintin Matheni.
Seth Puppywell - Seth is a secondary character of T.U.F.F. Puppies. He started his first day of T.U.F.F. with Connor and has been a good agent ever since. Seth used to be a comedian and to this day, he still tells many jokes.
Dudley Puppy - We all know who Dudley is. He is good partners with Connor and Seth in T.U.F.F. Puppies.
Quintin Matheni - Quintin Matheni is the main villain for the series. He is an evil dog that lives at Matheni Evil Inc. which is his HQ. Quintin despises T.U.F.F. and has an evil plan almost every day.
Alexis Jellyfish - She is Seth's love interest. She hasn't made an appearance in the show yet.
Jennifer Catstriever - She is Connor's love interest. She hasn't made an appearance in the show yet.
Charles the Henchman - Charles the Henchman is Quintin's henchman. He is very clumsy and often destroys Quintin Matheni Evil Inc.
Ronic the Hedgehog - He is a parody of Sonic the Hedgehog and makes numerous cameos in episodes. He is a citizen of Petropolis.
Perry Puppywell - He is Seth's brother. He will make an appearance in the show soon.
Biff Puppywell - She is Seth's sister.
Lisa Retriever - She is Connor's sister.
Joseph Retriever - He is Connor's brother. Joseph is seen in one episode with Connor.
Mrs. Matheni - Quintin's mom, who wants Quintin to stop being evil.
Mr. Retriever - Connor's father
Mrs. Retriever - Connor's mother
Mr. Puppywell - Seth's father
Mrs. Puppywell - Seth's mother
The Mathemagician - The greatest math expert in all of Petropolis.
Din the Bird - Seth's pet bird
Milo the Fish - Connor's betta fish who was tought many tricks (somehow)
Jeremy - Character who is often seen traveling with Connor.